Cyquant Nf Cell Proliferation Assay

patented CyQuant® GR Dye Assay Principle Page 3 3 Related picture) and quantified by CyQuant® GR Dye as described in the Assay Protocol

This protocol uses DMSO as a solubilizing agent for formazan to shorten the time of the assay Replace the culture medium

cyquant protocol A two-step warming protocol was shown to improve the cell recovery in this adherent cell model Frozen plates were removed from -135°C storage and placed

cyquant protocol polycarbonate membrane were detached and quantified by CyQuant® GR Dye as described in Assay Protocol Page 8 7 0 25 50 75 100 125 150 N IH 3T3 N